La panoplia de Brucellabúsqueda de efectores del Sistema de Secreción de Tipo IV y caracterización de inhibidores de lisozima

  1. Ortiz Pérez, Yelina
Supervised by:
  1. Matxalen Llosa Blas Director
  2. Félix Javier Sangari García Co-director

Defence university: Universidad de Cantabria

Fecha de defensa: 28 January 2020

Committee:
  1. Juan García Lobo Chair
  2. Raquel Conde Alvarez Secretary
  3. Suzana Pinto Salcedo Committee member

Type: Thesis

Teseo: 612395 DIALNET lock_openUCrea editor

Abstract

Brucella is an intracellular pathogen which has developed some mechanisms to face the adversities found inside the host. Among all the mechanisms of Brucella, we centered our study on the translocation of effector proteins to subvert the host cell and the inhibition of the host lysozyme activity. We have set up a new effector screening method based on assaying the interference with replication of the YFV. The screening showed 8 Brucella proteins that upregulate YFV and other 9 proteins that downregulate YFV. Moreover, it was showed that the Brucella protein named BAB1_0466 inhibits the lytic activity of lysozyme and is necessary for Brucella abortus survival in whole blood. In fact, BAB1_0466 could be important for B. abortus survival inside monocytes.