Recomendaciones preanalíticas para la obtención y análisis de ADN circulante a partir de sangre periférica. Recomendación (2018)

  1. Alvaro González Hernández
  2. Clara Pérez Barrios
  3. Hada C. Macher 1
  4. M. Sánchez Carbayo
  5. Antonio Barco Sánchez
  6. Antonio Fernández Suárez
  7. Antonio Martínez Peinado
  8. Blanca Ortiz Muñoz
  9. J. Trapé Pujol
  10. José Diego Santotoribio
  11. José María Augé Fradera 2
  12. María Jesús Gaspar Blázquez
  13. Rosa Carbonell Muñoz
  14. Xavier Filella 2
  1. 1 Comisión de Genética
  2. 2 Comisión de Marcadores Biológicos del Cáncer
Journal:
Revista del laboratorio clínico

ISSN: 1888-4008

Year of publication: 2019

Volume: 12

Issue: 3

Pages: 40-46

Type: Article

DOI: HTTPS://DOI.ORG/10.1016/J.LABCLI.2018.11.002 DIALNET GOOGLE SCHOLAR

More publications in: Revista del laboratorio clínico

Abstract

Cell-free DNA analysis in peripheral blood has been shown to be useful in oncology, organ transplantation, and prenatal screening. For its introduction into the clinical laboratory, it is necessary to ensure appropriate, reproducible and standardised pre-analytical protocols are in place. The aim of this document is to provide pre-analytical recommendations for obtaining of cell free DNA from peripheral blood. These recommendations include the type of sample and extraction tube, the method of centrifugation, the method for cell free DNA extraction, and measurement and storage conditions.

Data source: Dialnet