Potencial bioadhesivo de las nanopartículas de gliadina en el estómago

  1. Arangoa, Miguel Angel
  2. Campanero, Miguel Angel
  3. Irache, Juan
Zeitschrift:
Revista Colombiana de Ciencias Químico-Farmacéuticas

ISSN: 1909-6356 0034-7418

Datum der Publikation: 2004

Ausgabe: 33

Nummer: 1

Seiten: 38-47

Art: Artikel

Andere Publikationen in: Revista Colombiana de Ciencias Químico-Farmacéuticas

Zusammenfassung

The aim was to evaluate the bioadhesive potential of gliadin nanoparticles, either non-hardened (NP) or cross-linked with glutaraldehide (CL-NP), after their oral administration to laboratory animals. Nanoparticles were prepared by a desolvation method and using carbazol as fluorescent marker. This  method enabled us to obtain nanoparticles of about 450 nm and a carbazol loading of 12.5 mcg/mg. These carriers showed a great tropism for the upper gastrointestinal regions, mainly the stomach mucosa. This adhesive capacity may be explained by the gliadin composition with a high content in neutral and lipophilic residues. Neutral amino acids may promote hydrogen bonding interactions with the mucosa, while the lipophilic components may develop hydrophobic interactions with the biological support. Within the stomach, the amount of adhered nanoparticles to the glandular region was always significantly lower than to the non-glandular area. In this last region, CL-NP displayed a 1.5-times higher adhesive intensity than NP. Similarly, the mean residence time of the adhered fraction to the stomach mucosa was about 95 min higher for CL-NP than for NP. The strong bioadhesive interactions of gliadin nanoparticles with the stomach may be of interest for the treatment of local pathologies such as the eradication of Helicobacter pylori infections