Diagnóstico precoz de cáncer de pulmón en muestras de suero previas a su diagnóstico clínicoUtilidad de la metabolómica

  1. Antonio Pereira-Vega 1
  2. I. M. Díaz Olivares 2
  3. L. Padrón Fraysse 1
  4. G. Peces-Barba Romero 3
  5. L. Seijo Maceiras 4
  6. Carolina Gotera 3
  7. J.L. López-Campos Bodineau 5
  8. J.L. Gómez-Ariza 6
  9. Belén Callejón Leblic 6
  10. Tamara García Barrera 6
  1. 1 Servicio de Neumología del Hospital Juan Ramón Jiménerz (Huelva)
  2. 2 Fundación Andaluza Beturia de la Investigación en la Salud (FABIS)
  3. 3 Fundación Jiménez Díaz (Madrid)
  4. 4 Clínica Universidad de Navarra (Madrid)
  5. 5 Hospital Virgen del Rocío (Sevilla)
  6. 6 Departamento de Química Analítica de la Universidad de Huelva (UHU)
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Revue:
Revista española de patología torácica

ISSN: 1889-7347

Année de publication: 2024

Volumen: 36

Número: 2

Pages: 127-140

Type: Article

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D'autres publications dans: Revista española de patología torácica

Résumé

Introduction: New biomarkers (BM) based on omics techniques can help in the early diagnosis of lung cancer (LC). Our group has proposed 11 metabolites as possible BMs of CP. To validate these BMs, longitudinal studies are fundamental and rare. Objective: To analyze, in two retrospective longitudinal studies carried out in different cohorts, how the global metabolomic profile (GMP) varies in the years prior to the clinical diagnosis of PC. These cohorts follow up the included subjects for at least 5 years, with annual clinical visits and blood collection. Method and material: 72 samples have been selected from 38 subjects who have developed CP during follow-up. Baseline samples (time of diagnosis, n = 10) are compared with samples from 1 - 2 years (n = 25); 1 - 3 years (n = 38); and 4 - 7 years prior to the diagnosis of CP (n = 7). Advanced analytical chemistry techniques were used: mass spectrometry and liquid chromatography. For comparison between groups, Partial Least Squares Discriminant Analysis (PLS-DA) was used. Results: Significant differences were found in the comparisons of the PMG between the different groups. The causal metabolites of these differences and the metabolic pathways involved were determined, highlighting the metabolism of glycine, serine and threonine. They are compared with our 11 possible BMs. Conclusions: In the years prior to the clinical diagnosis of CP, metabolomic changes occur. There are metabolites that can be detected in the years prior to clinical diagnosis and that will provide very relevant data in the early diagnosis of PC.

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Artículo financiado con la Beca Neumosur nº 9/2015

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