Supporting data for "Genome-wide analysis of the H3K27me3 epigenome and transcriptome in Brassica rapa"

Resumen

Genome-wide maps of histone modifications have been obtained for several plant species. However, most studies focus on model systems and do not enforce FAIR data management principles. Here we study the H3K27me3 epigenome and associated transcriptome of Brassica rapa, an important vegetable cultivated world-wide. We obtained the genome-wide profile of the epigenetic mark H3K27me3 in B. rapa. We performed chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) from leaves and inflorescences. To correlate histone methylation and messenger RNA (mRNA) levels, we extracted RNA from the same harvested material and 3’-end mRNA high-throughput sequencing (3’RNA-seq) was performed. To facilitate transparency and reproducibility, a bioinformatic pipeline was generated for ChIP-seq and RNA-seq - the Reproducible Epigenomic pipeline. This pipeline has been made publicly available, together with the output data being extensively annotated in conformance with the FAIR data principles to allow transparency and reuse of our datasets and analytical tools. Our work reveals the epigenomic landscape of H3K27me3 in B. rapa and provide novel genomics datasets and bioinformatics analytical resources. We anticipate that this work will lead the way to further epigenomic studies in the complex genome of Brassica crops.