Florescent in situ hybridization with rDNA probes to the chromosomes of annual Brachypodium species.

  1. Trunova, Dana 1
  2. Borowska-Zuchowska, Natalia 1
  3. Mykhailyk, Serhii 1
  4. Xia, Kai 2
  5. Zhu, Yuanbin 2
  6. Sancho, Ruben 3
  7. Rojek-Jelonek, Magdalena 1
  8. Garcia, Sònia 4
  9. Wang, Kai 2
  10. Catalan, Pilar 3
  11. Kovarik, Ales 5
  12. Robert, Hasterok 1
  13. Kolano, Bożena 1
  1. 1 University of Silesia in Katowice
  2. 2 Nantong University
    info

    Nantong University

    Nantong, China

    ROR https://ror.org/02afcvw97

  3. 3 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

  4. 4 Institut Botànic de Barcelona
  5. 5 Czech Academy of Sciences
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Editor: RepOD

Ano de publicación: 2024

Tipo: Dataset

DOI: 10.18150/I7JHAO lock_openAcceso aberto editor

Resumo

The aim of the study was to draw a complex picture of rRNA gene evolutionary trajectories in two Brachypodium hybridum lineages of distinct evolutionary ages and cross directions. We aimed to address the following questions: (i) are the evolutionary trajectories of 35S and 5S rDNA homoeologues similar or not?; (ii) is there a relationship between the direction of the cross and rDNA homoeologues loss in B. hybridum?; (iii) do the rRNA genes in the studied B. hybridum lineages of different evolutionary ages follow the same evolutionary pathways?Description of the database: Microphotographs (TIF files) of mitotic metaphase chromosomes after fluorescence in situ hybridisation (FISH) with 25S rDNA and 5S rDNA as probes. All microphotographs were acquired using a Zeiss AxioImager.Z.2 fluorescent microscope equipped with an AxioCamHMr camera (Zeiss, Oberkochen, Germany). The microphotographs were processed uniformly using ZEN 2.3 Pro (Zeiss). Number of TIF files: 12.